Hybrid-denovo: a de novo OTU-picking pipeline integrating single-end and paired-end 16S sequence tags

BACKGROUND: Illumina paired-end sequencing has been increasingly popular for 16S rRNA gene-based microbiota profiling. It provides higher phylogenetic resolution than single-end reads due to a longer read length. However, the reverse read (R2) often has significant low base quality, and a large prop...

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Vydáno v:Gigascience
Hlavní autoři: Chen, Xianfeng, Johnson, Stephen, Jeraldo, Patricio, Wang, Junwen, Chia, Nicholas, Kocher, Jean-Pierre A, Chen, Jun
Médium: Artigo
Jazyk:Inglês
Vydáno: Oxford University Press 2017
Témata:
Technical Note
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC5841375/
https://ncbi.nlm.nih.gov/pubmed/29267858
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/gigascience/gix129
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