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Effect of Plasmid Design and Type of Integration Event on Recombinant Protein Expression in Pichia pastoris
Pichia pastoris (syn. Komagataella phaffii) is one of the most common eukaryotic expression systems for heterologous protein production. Expression cassettes are typically integrated in the genome to obtain stable expression strains. In contrast to Saccharomyces cerevisiae, where short overhangs are...
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| Опубликовано в: : | Appl Environ Microbiol |
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| Главные авторы: | , , , |
| Формат: | Artigo |
| Язык: | Inglês |
| Опубликовано: |
American Society for Microbiology
2018
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| Предметы: | |
| Online-ссылка: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5835746/ https://ncbi.nlm.nih.gov/pubmed/29330186 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.02712-17 |
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