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In Vivo Functional Assay of a Recombinant Aquaporin in Pichia pastoris

The water channel protein PvTIP3;1 (α-TIP) is a member of the major intrinsic protein (MIP) membrane channel family. We overexpressed this eukaryotic aquaporin in the methylotrophic yeast Pichia pastoris, and immunogold labeling of cellular cryosections showed that the protein accumulated in the pla...

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Detalhes bibliográficos
Main Authors: Daniels, Mark J., Wood, Malcolm R., Yeager, Mark
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology 2006
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC1392912/
https://ncbi.nlm.nih.gov/pubmed/16461705
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.72.2.1507-1514.2006
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