A DGCR8-Independent Stable MicroRNA Expression Strategy Reveals Important Functions of miR-290 and miR-183–182 Families in Mouse Embryonic Stem Cells

Dgcr8 knockout cells provide a great means to understand the function of microRNAs (miRNAs) in vitro and in vivo. Current strategies to study miRNA function in Dgcr8 knockout cells depend on transient transfection of chemically synthesized miRNA mimics, which is costly and not suitable for long-term...

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Vydáno v:Stem Cell Reports
Hlavní autoři: Wang, Xi-Wen, Hao, Jing, Guo, Wen-Ting, Liao, Le-Qi, Huang, Si-Yue, Guo, Xiangpeng, Bao, Xichen, Esteban, Miguel A., Wang, Yangming
Médium: Artigo
Jazyk:Inglês
Vydáno: Elsevier 2017
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Article
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC5830984/
https://ncbi.nlm.nih.gov/pubmed/28988987
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.stemcr.2017.08.027
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