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Extraction of total RNA from single-oocytes and single-cell mRNA sequencing of swine oocytes
OBJECTIVE: Analyses of single oocytes are essential for a fine dissection of molecular features governing developmental competence. We adapted the phenol–chloroform procedure for the purification of total RNA from single oocytes. RESULTS: Key modifications include the use of Phasemaker™ tubes, a sec...
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| Publicado no: | BMC Res Notes |
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| Main Authors: | , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
BioMed Central
2018
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5828076/ https://ncbi.nlm.nih.gov/pubmed/29482623 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/s13104-018-3264-2 |
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