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miPrimer: an empirical-based qPCR primer design method for small noncoding microRNA
MicroRNAs (miRNAs) are 18–25 nucleotides (nt) of highly conserved, noncoding RNAs involved in gene regulation. Because of miRNAs’ short length, the design of miRNA primers for PCR amplification remains a significant challenge. Adding to the challenge are miRNAs similar in sequence and miRNA family m...
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| Veröffentlicht in: | RNA |
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| Hauptverfasser: | , , , , , |
| Format: | Artigo |
| Sprache: | Inglês |
| Veröffentlicht: |
Cold Spring Harbor Laboratory Press
2018
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| Schlagworte: | |
| Online Zugang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5824350/ https://ncbi.nlm.nih.gov/pubmed/29208706 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1261/rna.061150.117 |
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