Efficient and Scalable Precision Genome Editing in Staphylococcus aureus through Conditional Recombineering and CRISPR/Cas9-Mediated Counterselection

Staphylococcus aureus is an important human pathogen, but studies of the organism have suffered from the lack of a robust tool set for its genetic and genomic manipulation. Here we report the development of a system for the facile and high-throughput genomic engineering of S. aureus using single-str...

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Veröffentlicht in:mBio
Hauptverfasser: Penewit, Kelsi, Holmes, Elizabeth A., McLean, Kathyrn, Ren, Mingxin, Waalkes, Adam, Salipante, Stephen J.
Format: Artigo
Sprache:Inglês
Veröffentlicht: American Society for Microbiology 2018
Schlagworte:
Research Article
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC5821094/
https://ncbi.nlm.nih.gov/pubmed/29463653
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/mBio.00067-18
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