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Application of methyl-TROSY to a large paramagnetic membrane protein without perdeuteration: (13)C-MMTS-labeled NADPH-cytochrome P450 oxidoreductase
NMR spectroscopy of membrane proteins involved in electron transport is difficult due to the presence of both the lipids and paramagnetic centers. Here we report the solution NMR study of the NADPH-cytochrome P450 oxidoreductase (POR) in its reduced and oxidized states. We interrogate POR, first, in...
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| Publicado no: | J Biomol NMR |
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| Main Authors: | , , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2017
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5820150/ https://ncbi.nlm.nih.gov/pubmed/29168021 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/s10858-017-0152-3 |
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