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FACS isolation of live mouse eosinophils at high purity via a protocol that does not target Siglec F
Flow cytometry protocols designed to identify mouse eosinophils typically target Siglec F, an α-2,3-sialic acid binding transmembrane protein expressed universally on cells of this lineage. While a convenient target, antibody-mediated ligation of Siglec F induces eosinophil apoptosis, which limits i...
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| Опубликовано в: : | J Immunol Methods |
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| Главные авторы: | , , |
| Формат: | Artigo |
| Язык: | Inglês |
| Опубликовано: |
2017
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| Предметы: | |
| Online-ссылка: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5818306/ https://ncbi.nlm.nih.gov/pubmed/29253503 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.jim.2017.12.001 |
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