Cas9-mediated excision of proximal DNaseI/H3K4me3 signatures confers robust silencing of microRNA and long non-coding RNA genes

CRISPR/Cas9-based approaches have greatly facilitated targeted genomic deletions. Contrary to coding genes however, which can be functionally knocked out by frame-shift mutagenesis, non-coding RNA (ncRNA) gene knockouts have remained challenging. Here we present a universal ncRNA knockout approach g...

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Vydáno v:PLoS One
Hlavní autoři: Janga, Harshavardhan, Aznaourova, Marina, Boldt, Fabian, Damm, Katrin, Grünweller, Arnold, Schulte, Leon N.
Médium: Artigo
Jazyk:Inglês
Vydáno: Public Library of Science 2018
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Research Article
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC5815609/
https://ncbi.nlm.nih.gov/pubmed/29451908
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0193066
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