A novel D(2)O tracer method to quantify RNA turnover as a biomarker of de novo ribosomal biogenesis, in vitro, in animal models, and in human skeletal muscle

Current methods to quantify in vivo RNA dynamics are limited. Here, we developed a novel stable isotope (D(2)O) methodology to quantify RNA synthesis (i.e., ribosomal biogenesis) in cells, animal models, and humans. First, proliferating C2C12 cells were incubated in D(2)O-enriched media and myotubes...

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Enregistré dans:
Détails bibliographiques
Publié dans:Am J Physiol Endocrinol Metab
Auteurs principaux: Brook, M. S., Wilkinson, D. J., Mitchell, W. K., Lund, J. L., Phillips, B. E., Szewczyk, N. J., Kainulainen, H., Lensu, S., Koch, L. G., Britton, S. L., Greenhaff, P. L., Smith, K., Atherton, P. J.
Format: Artigo
Langue:Inglês
Publié: American Physiological Society 2017
Sujets:
Innovative Methodology
Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC5814597/
https://ncbi.nlm.nih.gov/pubmed/28811296
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1152/ajpendo.00157.2017
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