Automation aided optimization of cloning, expression and purification of enzymes of the bacterial sialic acid catabolic and sialylation pathways enzymes for structural studies

The process of obtaining a well‐expressing, soluble and correctly folded constructs can be made easier and quicker by automating the optimization of cloning, expression and purification. While there are many semiautomated pipelines available for cloning, expression and purification, there is hardly...

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Bibliografski detalji
Izdano u:Microb Biotechnol
Glavni autori: Bairy, Sneha, Gopalan, Lakshmi Narayanan, Setty, Thanuja Gangi, Srinivasachari, Sathya, Manjunath, Lavanyaa, Kumar, Jay Prakash, Guntupalli, Sai R, Bose, Sucharita, Nayak, Vinod, Ghosh, Swagatha, Sathyanarayanan, Nitish, Caing‐Carlsson, Rhawnie, Wahlgren, Weixiao Yuan, Friemann, Rosmarie, Ramaswamy, S., Neerathilingam, Muniasamy
Format: Artigo
Jezik:Inglês
Izdano: John Wiley and Sons Inc. 2018
Teme:
Brief Report
Online pristup:https://ncbi.nlm.nih.gov/pmc/articles/PMC5812244/
https://ncbi.nlm.nih.gov/pubmed/29345069
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1111/1751-7915.13041
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