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Automation aided optimization of cloning, expression and purification of enzymes of the bacterial sialic acid catabolic and sialylation pathways enzymes for structural studies

The process of obtaining a well‐expressing, soluble and correctly folded constructs can be made easier and quicker by automating the optimization of cloning, expression and purification. While there are many semiautomated pipelines available for cloning, expression and purification, there is hardly...

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Detalhes bibliográficos
Publicado no:Microb Biotechnol
Main Authors: Bairy, Sneha, Gopalan, Lakshmi Narayanan, Setty, Thanuja Gangi, Srinivasachari, Sathya, Manjunath, Lavanyaa, Kumar, Jay Prakash, Guntupalli, Sai R, Bose, Sucharita, Nayak, Vinod, Ghosh, Swagatha, Sathyanarayanan, Nitish, Caing‐Carlsson, Rhawnie, Wahlgren, Weixiao Yuan, Friemann, Rosmarie, Ramaswamy, S., Neerathilingam, Muniasamy
Formato: Artigo
Idioma:Inglês
Publicado em: John Wiley and Sons Inc. 2018
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5812244/
https://ncbi.nlm.nih.gov/pubmed/29345069
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1111/1751-7915.13041
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