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Isolation of Amyloid Plaques and Neurofibrillary Tangles from Archived Alzheimer’s Disease Tissue Using Laser-Capture Microdissection for Downstream Proteomics

Here, we describe a new method that allows localized proteomics of amyloid plaques and neurofibrillary tangles (NFTs), which are the two pathological hallmarks of Alzheimer’s disease (AD). Amyloid plaques and NFTs are visualized using immunohistochemistry and microdissected from archived, formalin-f...

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Detalhes bibliográficos
Publicado no:Methods Mol Biol
Main Authors: Drummond, Eleanor, Nayak, Shruti, Pires, Geoffrey, Ueberheide, Beatrix, Wisniewski, Thomas
Formato: Artigo
Idioma:Inglês
Publicado em: 2018
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5811767/
https://ncbi.nlm.nih.gov/pubmed/29344869
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/978-1-4939-7558-7_18
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