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Single cell on-chip whole genome amplification via micropillar arrays for reduced amplification bias

Single cell whole genome amplification is susceptible to amplification biases that impact the accuracy of single cell sequencing data. To address this, we have developed a microfluidic device for the isolation and purification of genomic DNA from individual cells. The device uses a micropillar array...

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Bibliographische Detailangaben
Veröffentlicht in:PLoS One
Hauptverfasser: Tian, Harvey C., Benitez, Jaime J., Craighead, Harold G.
Format: Artigo
Sprache:Inglês
Veröffentlicht: Public Library of Science 2018
Schlagworte:
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC5809021/
https://ncbi.nlm.nih.gov/pubmed/29432426
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0191520
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