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Illumina Library Preparation for Sequencing the GC-Rich Fraction of Heterogeneous Genomic DNA

Standard Illumina libraries are biased toward sequences of intermediate GC-content. This results in an underrepresentation of GC-rich regions in sequencing projects of genomes with heterogeneous base composition, such as mammals and birds. We developed a simple, cost-effective protocol to enrich she...

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Dettagli Bibliografici
Pubblicato in:Genome Biol Evol
Autori principali: Tilak, Marie-Ka, Botero-Castro, Fidel, Galtier, Nicolas, Nabholz, Benoit
Natura: Artigo
Lingua:Inglês
Pubblicazione: Oxford University Press 2018
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Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC5808798/
https://ncbi.nlm.nih.gov/pubmed/29385572
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/gbe/evy022
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