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Highly efficient generation of knock-in transgenic medaka by CRISPR/Cas9-mediated genome engineering
BACKGROUND: Medaka (Oryzias latipes) is a popular animal model used in vertebrate genetic analysis. Recently, an efficient (~ 30%) knock-in system via non-homologous end joining (NHEJ) was established in zebrafish using the CRISPR/Cas9 system. If the same technique were applicable in medaka, it woul...
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| Publicado no: | Zoological Lett |
|---|---|
| Main Authors: | , , , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
BioMed Central
2018
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5798193/ https://ncbi.nlm.nih.gov/pubmed/29445519 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/s40851-017-0086-3 |
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