The Effect of DNA Extraction Methods on Observed Microbial Communities from Fibrous and Liquid Rumen Fractions of Dairy Cows

DNA based methods have been widely used to study the complexity of the rumen microbiota, and it is well known that the method of DNA extraction is a critical step in enabling accurate assessment of this complexity. Rumen fluid (RF) and fibrous content (FC) fractions differ substantially in terms of...

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Detalhes bibliográficos
Publicado no:Front Microbiol
Main Authors: Vaidya, Jueeli D., van den Bogert, Bartholomeus, Edwards, Joan E., Boekhorst, Jos, van Gastelen, Sanne, Saccenti, Edoardo, Plugge, Caroline M., Smidt, Hauke
Formato: Artigo
Idioma:Inglês
Publicado em: Frontiers Media S.A. 2018
Assuntos:
Microbiology
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5797766/
https://ncbi.nlm.nih.gov/pubmed/29445366
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3389/fmicb.2018.00092
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