The Effect of DNA Extraction Methods on Observed Microbial Communities from Fibrous and Liquid Rumen Fractions of Dairy Cows

DNA based methods have been widely used to study the complexity of the rumen microbiota, and it is well known that the method of DNA extraction is a critical step in enabling accurate assessment of this complexity. Rumen fluid (RF) and fibrous content (FC) fractions differ substantially in terms of...

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Vydáno v:Front Microbiol
Hlavní autoři: Vaidya, Jueeli D., van den Bogert, Bartholomeus, Edwards, Joan E., Boekhorst, Jos, van Gastelen, Sanne, Saccenti, Edoardo, Plugge, Caroline M., Smidt, Hauke
Médium: Artigo
Jazyk:Inglês
Vydáno: Frontiers Media S.A. 2018
Témata:
Microbiology
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC5797766/
https://ncbi.nlm.nih.gov/pubmed/29445366
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3389/fmicb.2018.00092
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