Two approaches for estimating the lower limit of quantitation (LLOQ) of microRNA levels assayed as exploratory biomarkers by RT-qPCR

BACKGROUND: Circulating microRNAs are undergoing exploratory use as safety biomarkers in drug development. Reverse transcription quantitative polymerase chain reaction (RT-qPCR) is one common approach used to quantitate levels of microRNAs in samples that includes the use of a standard curve of cali...

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Veröffentlicht in:BMC Biotechnol
Hauptverfasser: Wolfinger, Russell D., Beedanagari, Sudheer, Boitier, Eric, Chen, Tao, Couttet, Philippe, Ellinger-Ziegelbauer, Heidrun, Guillemain, Gregory, Mariet, Claire, Mouritzen, Peter, O’Lone, Raegan, Pine, P. Scott, Sharapova, Tatiana, Yan, Jian, Yuen, Peter S., Thompson, Karol L.
Format: Artigo
Sprache:Inglês
Veröffentlicht: BioMed Central 2018
Schlagworte:
Methodology Article
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC5796571/
https://ncbi.nlm.nih.gov/pubmed/29391006
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/s12896-018-0415-4
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