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Determination of ergocalciferol in human plasma after Diels-Alder derivatization by LC–MS/MS and its application to a bioequivalence study
An accurate, sensitive and selective method is developed for determination of ergocalciferol (vitamin D(2)) in human plasma using LC–MS/MS. After liquid-liquid extraction with n-hexane, ergocalciferol was derivatized by reacting with 4-phenyl-1,2,4-triazoline-3,5-dione (PTAD), a strong dienophile ba...
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| Publié dans: | J Pharm Anal |
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| Auteurs principaux: | , , , , |
| Format: | Artigo |
| Langue: | Inglês |
| Publié: |
Xi'an Jiaotong University
2017
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| Sujets: | |
| Accès en ligne: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5790750/ https://ncbi.nlm.nih.gov/pubmed/29404069 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.jpha.2017.05.006 |
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