Determination of ergocalciferol in human plasma after Diels-Alder derivatization by LC–MS/MS and its application to a bioequivalence study

An accurate, sensitive and selective method is developed for determination of ergocalciferol (vitamin D(2)) in human plasma using LC–MS/MS. After liquid-liquid extraction with n-hexane, ergocalciferol was derivatized by reacting with 4-phenyl-1,2,4-triazoline-3,5-dione (PTAD), a strong dienophile ba...

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Detalhes bibliográficos
Publicado no:J Pharm Anal
Main Authors: Contractor, Pritesh, Gandhi, Abhishek, Solanki, Gajendra, Shah, Priyanka A., Shrivastav, Pranav S.
Formato: Artigo
Idioma:Inglês
Publicado em: Xi'an Jiaotong University 2017
Assuntos:
Short Communication
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5790750/
https://ncbi.nlm.nih.gov/pubmed/29404069
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.jpha.2017.05.006
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