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Advantages of meta-total RNA sequencing (MeTRS) over shotgun metagenomics and amplicon-based sequencing in the profiling of complex microbial communities

Sequencing-based microbiome profiling aims at detecting and quantifying individual members of a microbial community in a culture-independent manner. While amplicon-based sequencing (ABS) of bacterial or fungal ribosomal DNA is the most widely used technology due to its low cost, it suffers from PCR...

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Vydáno v:NPJ Biofilms Microbiomes
Hlavní autoři: Cottier, Fabien, Srinivasan, Kandhadayar Gopalan, Yurieva, Marina, Liao, Webber, Poidinger, Michael, Zolezzi, Francesca, Pavelka, Norman
Médium: Artigo
Jazyk:Inglês
Vydáno: Nature Publishing Group UK 2018
Témata:
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC5773663/
https://ncbi.nlm.nih.gov/pubmed/29367879
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41522-017-0046-x
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