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Visualization of barriers and obstacles to molecular diffusion in live cells by spatial pair-cross-correlation in two dimensions

Despite recent advances in optical super-resolution, we lack a method that can visualize the path followed by diffusing molecules in the cytoplasm or in the nucleus of cells. Fluorescence correlation spectroscopy (FCS) provides molecular dynamics at the single molecule level by averaging the behavio...

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Bibliographic Details
Published in:Biomed Opt Express
Main Authors: Malacrida, Leonel, Hedde, Per Niklas, Ranjit, Suman, Cardarelli, Francesco, Gratton, Enrico
Format: Artigo
Language:Inglês
Published: Optical Society of America 2017
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Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC5772584/
https://ncbi.nlm.nih.gov/pubmed/29359105
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1364/BOE.9.000303
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