Distinguishing signal from autofluorescence in cryogenic correlated light and electron microscopy of mammalian cells

In cryogenic correlated light and electron microscopy (cryo-CLEM), frozen targets of interest are identified and located on EM grids by fluorescence microscopy and then imaged at higher resolution by cryo-EM. Whilst working with these methods, we discovered that a variety of mammalian cells exhibit...

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Detalhes bibliográficos
Publicado no:J Struct Biol
Main Authors: Carter, Stephen D., Mageswaran, Shrawan K., Farino, Zachary J., Mamede, João I., Oikonomou, Catherine M., Hope, Thomas J., Freyberg, Zachary, Jensen, Grant J.
Formato: Artigo
Idioma:Inglês
Publicado em: 2017
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Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5771259/
https://ncbi.nlm.nih.gov/pubmed/29078993
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.jsb.2017.10.009
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