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High-aperture cryogenic light microscopy
We report here the development of instruments and protocols for carrying out high numerical aperture immersion light microscopy on cryogenic specimens. Imaging by this modality greatly increases the lifetimes of fluorescence probes, including those commonly used for protein localization studies, whi...
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| Main Authors: | , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2009
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3350647/ https://ncbi.nlm.nih.gov/pubmed/19566622 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1111/j.1365-2818.2009.03184.x |
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