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Engineering of Bacteriophage T4 Genome Using CRISPR-Cas9

Bacteriophages likely constitute the largest biomass on Earth. However, very few phage genomes have been well-characterized, the tailed phage T4 genome being one of them. Even in T4, much of the genome remained uncharacterized. The classical genetic strategies are tedious, compounded by genome modif...

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Detalhes bibliográficos
Publicado no:ACS Synth Biol
Main Authors: Tao, Pan, Wu, Xiaorong, Tang, Wei-Chun, Zhu, Jingen, Rao, Venigalla
Formato: Artigo
Idioma:Inglês
Publicado em: 2017
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5771229/
https://ncbi.nlm.nih.gov/pubmed/28657724
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/acssynbio.7b00179
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