Profiling protein S-sulfination with maleimide-linked probes

Cysteine residues are susceptible to oxidation to form S-sulfinyl (R-SO(2)H) and S-sulfonyl (R-SO(3)H) post-translational modifications. Here we present a simple bioconjugation strategy to label S-sulfinated proteins using reporter-linked maleimides. After alkylation of free thiols with iodoacetamid...

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Udgivet i:Chembiochem
Main Authors: Kuo, Yu-Hsuan, Konopko, Aaron M., Borotto, Nicholas B., Majmudar, Jaimeen D., Haynes, Sarah E., Martin, Brent R.
Format: Artigo
Sprog:Inglês
Udgivet: 2017
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Article
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC5765543/
https://ncbi.nlm.nih.gov/pubmed/28809078
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/cbic.201700137
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