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Site-specific randomization of the endogenous genome by a regulatable CRISPR-Cas9 piggyBac system in human cells
Randomized mutagenesis at an endogenous chromosomal locus is a promising approach for protein engineering, functional assessment of regulatory elements, and modeling genetic variations. In mammalian cells, however, it is challenging to perform site-specific single-nucleotide substitution with single...
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| Опубликовано в: : | Sci Rep |
|---|---|
| Главные авторы: | , , , , , , |
| Формат: | Artigo |
| Язык: | Inglês |
| Опубликовано: |
Nature Publishing Group UK
2018
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| Предметы: | |
| Online-ссылка: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5762678/ https://ncbi.nlm.nih.gov/pubmed/29321585 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41598-017-18568-4 |
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