Á lódáil...

Site-specific randomization of the endogenous genome by a regulatable CRISPR-Cas9 piggyBac system in human cells

Randomized mutagenesis at an endogenous chromosomal locus is a promising approach for protein engineering, functional assessment of regulatory elements, and modeling genetic variations. In mammalian cells, however, it is challenging to perform site-specific single-nucleotide substitution with single...

Cur síos iomlán

Na minha lista:
Sonraí Bibleagrafaíochta
Foilsithe in:Sci Rep
Main Authors: Ishida, Kentaro, Xu, Huaigeng, Sasakawa, Noriko, Lung, Mandy Siu Yu, Kudryashev, Julia Alexandra, Gee, Peter, Hotta, Akitsu
Formáid: Artigo
Teanga:Inglês
Foilsithe: Nature Publishing Group UK 2018
Ábhair:
Rochtain Ar Líne:https://ncbi.nlm.nih.gov/pmc/articles/PMC5762678/
https://ncbi.nlm.nih.gov/pubmed/29321585
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41598-017-18568-4
Clibeanna: Cuir Clib Leis
Gan Chlibeanna, Bí ar an gcéad duine leis an taifead seo a chlibeáil!