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A General Method for Intracellular Protein Delivery through ‘E-tag’ Protein Engineering and Arginine Functionalized Gold Nanoparticles

In this protocol, we describe a method for direct cytosolic protein delivery that avoids endosomal entrapment of the delivered proteins. We achieved this by tagging the desired protein with an oligo glutamic acid tag (E-tag), and subsequently using carrier gold nanoparticles to deliver these E-tagge...

詳細記述

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書誌詳細
出版年:Bio Protoc
主要な著者: Mout, Rubul, Rotello, Vincent M.
フォーマット: Artigo
言語:Inglês
出版事項: Bio-Protocol 2017
主題:
オンライン・アクセス:https://ncbi.nlm.nih.gov/pmc/articles/PMC5760992/
https://ncbi.nlm.nih.gov/pubmed/29333478
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.21769/BioProtoc.2661
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