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Advanced Confocal Microscopy Techniques to Study Protein-protein Interactions and Kinetics at DNA Lesions

Local microirradiation with lasers represents a useful tool for studies of DNA-repair-related processes in live cells. Here, we describe a methodological approach to analyzing protein kinetics at DNA lesions over time or protein-protein interactions on locally microirradiated chromatin. We also show...

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Detalhes bibliográficos
Publicado no:J Vis Exp
Main Authors: Legartová, Soňa, Suchánková, Jana, Krejčí, Jana, Kovaříková, Alena, Bártová, Eva
Formato: Artigo
Idioma:Inglês
Publicado em: MyJove Corporation 2017
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5755375/
https://ncbi.nlm.nih.gov/pubmed/29155761
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/55999
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