Glyoxal as an alternative fixative to formaldehyde in immunostaining and super‐resolution microscopy

Paraformaldehyde (PFA) is the most commonly used fixative for immunostaining of cells, but has been associated with various problems, ranging from loss of antigenicity to changes in morphology during fixation. We show here that the small dialdehyde glyoxal can successfully replace PFA. Despite being...

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Detalhes bibliográficos
Publicado no:EMBO J
Main Authors: Richter, Katharina N, Revelo, Natalia H, Seitz, Katharina J, Helm, Martin S, Sarkar, Deblina, Saleeb, Rebecca S, D'Este, Elisa, Eberle, Jessica, Wagner, Eva, Vogl, Christian, Lazaro, Diana F, Richter, Frank, Coy‐Vergara, Javier, Coceano, Giovanna, Boyden, Edward S, Duncan, Rory R, Hell, Stefan W, Lauterbach, Marcel A, Lehnart, Stephan E, Moser, Tobias, Outeiro, Tiago F, Rehling, Peter, Schwappach, Blanche, Testa, Ilaria, Zapiec, Bolek, Rizzoli, Silvio O
Formato: Artigo
Idioma:Inglês
Publicado em: John Wiley and Sons Inc. 2017
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Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5753035/
https://ncbi.nlm.nih.gov/pubmed/29146773
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.15252/embj.201695709
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