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Real-time Quaking-induced Conversion Assay for Detection of CWD Prions in Fecal Material

The RT-QuIC technique is a sensitive in vitro cell-free prion amplification assay based mainly on the seeded misfolding and aggregation of recombinant prion protein (PrP) substrate using prion seeds as a template for the conversion. RT-QuIC is a novel high-throughput technique which is analogous to...

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Detalhes bibliográficos
Publicado no:J Vis Exp
Main Authors: Cheng, Yo Ching, Hannaoui, Samia, John, Theodore Ralph, Dudas, Sandor, Czub, Stefanie, Gilch, Sabine
Formato: Artigo
Idioma:Inglês
Publicado em: MyJove Corporation 2017
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5752360/
https://ncbi.nlm.nih.gov/pubmed/28994814
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/56373
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