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Real-time Quaking-induced Conversion Assay for Detection of CWD Prions in Fecal Material
The RT-QuIC technique is a sensitive in vitro cell-free prion amplification assay based mainly on the seeded misfolding and aggregation of recombinant prion protein (PrP) substrate using prion seeds as a template for the conversion. RT-QuIC is a novel high-throughput technique which is analogous to...
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Publicado no: | J Vis Exp |
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Main Authors: | , , , , , |
Formato: | Artigo |
Idioma: | Inglês |
Publicado em: |
MyJove Corporation
2017
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Assuntos: | |
Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5752360/ https://ncbi.nlm.nih.gov/pubmed/28994814 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/56373 |
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