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In Situ MHC-tetramer Staining and Quantitative Analysis to Determine the Location, Abundance, and Phenotype of Antigen-specific CD8 T Cells in Tissues

T cells are critical to many immunological processes, including detecting and eliminating virus-infected cells, preventing autoimmunity, assisting in B-cell and plasma-cell production of antibodies, and detecting and eliminating cancer cells. The development of MHC-tetramer staining of antigen-speci...

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Detalhes bibliográficos
Publicado no:J Vis Exp
Main Authors: Li, Shengbin, Mwakalundwa, Gwantwa, Skinner, Pamela J.
Formato: Artigo
Idioma:Inglês
Publicado em: MyJove Corporation 2017
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5752327/
https://ncbi.nlm.nih.gov/pubmed/28994787
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/56130
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