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Visualizing Antigen Specific CD4+ T Cells using MHC Class II Tetramers

Major histocompatibility complex (MHC) class II tetramers allow the direct visualization of antigen specific CD4+ T cells by flow cytometry. This method relies on the highly specific interaction between peptide loaded MHC and the corresponding T-cell receptor. While the affinity of a single MHC/pept...

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Detalhes bibliográficos
Main Authors: James, Eddie A., LaFond, Rebecca, Durinovic-Bello, Ivana, Kwok, William
Formato: Artigo
Idioma:Inglês
Publicado em: MyJove Corporation 2009
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2789100/
https://ncbi.nlm.nih.gov/pubmed/19270641
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/1167
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