Accepting from the best donor; analysis of long-lifetime donor fluorescent protein pairings to optimise dynamic FLIM-based FRET experiments

FRET biosensors have proven very useful tools for studying the activation of specific signalling pathways in living cells. Most biosensors designed to date have been predicated on fluorescent protein pairs that were identified by, and for use in, intensity based measurements, however fluorescence li...

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Bibliografski detalji
Izdano u:PLoS One
Glavni autori: Martin, Kirsty J., McGhee, Ewan J., Schwarz, Juliana P., Drysdale, Martin, Brachmann, Saskia M., Stucke, Volker, Sansom, Owen J., Anderson, Kurt I.
Format: Artigo
Jezik:Inglês
Izdano: Public Library of Science 2018
Teme:
Research Article
Online pristup:https://ncbi.nlm.nih.gov/pmc/articles/PMC5749721/
https://ncbi.nlm.nih.gov/pubmed/29293509
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0183585
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