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Haplotype-Contained PCR Products Analysis by Sequencing with Selective Restriction of Primer Extension

We develop a strategy for haplotype analysis of PCR products that contained two adjacent heterozygous loci using sequencing with specific primers, allele-specific primers, and ddNTP-blocked primers. To validate its feasibility, two sets of PCR products, including two adjacent heterozygous SNPs, UGT1...

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Detalhes bibliográficos
Publicado no:Biomed Res Int
Main Authors: Wang, Liu, Xiao, Pengfeng
Formato: Artigo
Idioma:Inglês
Publicado em: Hindawi 2017
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5742430/
https://ncbi.nlm.nih.gov/pubmed/29376065
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1155/2017/1397902
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