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Degradation of RNA during lysis of Escherichia coli cells in agarose plugs breaks the chromosome

The nucleoid of Escherichia coli comprises DNA, nucleoid associated proteins (NAPs) and RNA, whose role is unclear. We found that lysing bacterial cells embedded in agarose plugs in the presence of RNases caused massive fragmentation of the chromosomal DNA. This RNase-induced chromosomal fragmentati...

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Bibliografski detalji
Izdano u:PLoS One
Glavni autori: Khan, Sharik R., Kuzminov, Andrei
Format: Artigo
Jezik:Inglês
Izdano: Public Library of Science 2017
Teme:
Online pristup:https://ncbi.nlm.nih.gov/pmc/articles/PMC5739488/
https://ncbi.nlm.nih.gov/pubmed/29267353
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0190177
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