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Arrayed mutant haploid embryonic stem cell libraries facilitate phenotype-driven genetic screens

Forward genetic screens using mammalian embryonic stem (ES) cells have identified genes required for numerous cellular processes. However, loss-of-function screens are more difficult to conduct in diploid cells because, in most cases, both alleles of a gene must be mutated to exhibit a phenotype. Re...

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Detalhes bibliográficos
Publicado no:Nucleic Acids Res
Main Authors: Liu, Guang, Wang, Xue, Liu, Yufang, Zhang, Meili, Cai, Tao, Shen, Zhirong, Jia, Yuyan, Huang, Yue
Formato: Artigo
Idioma:Inglês
Publicado em: Oxford University Press 2017
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5727442/
https://ncbi.nlm.nih.gov/pubmed/29036617
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkx857
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