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Amplification of nonspecific products in quantitative polymerase chain reactions (qPCR)

Quantitative PCR allows the precise measurement of DNA concentrations and is generally considered to be straightforward and trouble free. However, a survey with 93 validated assays for genes in the Wnt-pathway showed that the amplification of nonspecific products occurs frequently and is unrelated t...

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Wedi'i Gadw mewn:
Manylion Llyfryddiaeth
Cyhoeddwyd yn:Biomol Detect Quantif
Prif Awduron: Ruiz-Villalba, Adrián, van Pelt-Verkuil, Elizabeth, Gunst, Quinn D, Ruijter, Jan M, van den Hoff, Maurice JB
Fformat: Artigo
Iaith:Inglês
Cyhoeddwyd: Elsevier 2017
Pynciau:
Mynediad Ar-lein:https://ncbi.nlm.nih.gov/pmc/articles/PMC5727009/
https://ncbi.nlm.nih.gov/pubmed/29255685
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.bdq.2017.10.001
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