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Exploiting Uniformly (13)C-Labeled Carbohydrates for Probing Carbohydrate-Protein Interactions by NMR Spectroscopy

NMR of a uniformly (13)C-labeled carbohydrate was used to elucidate the atomic details of a sugar-protein complex. The structure of the (13)C-labeled Manα(1–2)Manα(1–2)ManαOMe trisaccharide ligand when bound to cyanovirin-N was characterized and revealed that in the complex the glycosidic linkage to...

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Publicado en:J Am Chem Soc
Main Authors: Nestor, Gustav, Anderson, Taigh, Oscarson, Stefan, Gronenborn, Angela M.
Formato: Artigo
Idioma:Inglês
Publicado: 2017
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Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC5725960/
https://ncbi.nlm.nih.gov/pubmed/28406013
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/jacs.7b01929
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