Screening and Detecting Salmonella in Different Food Matrices in Southern Tunisia Using a Combined Enrichment/Real-Time PCR Method: Correlation with Conventional Culture Method

A combined enrichment/ newly developed invA TaqMan(®) real-time PCR (qPCR) method as a screening assay to detect Salmonella spp. in 500 naturally food matrices is evaluated. DNA template for qPCR was extracted from an overnight pre-enriched sample in buffered peptone water using lysis–guanidine isot...

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Bibliographische Detailangaben
Veröffentlicht in:Front Microbiol
Hauptverfasser: Siala, Mariam, Barbana, Amina, Smaoui, Salma, Hachicha, Salma, Marouane, Chema, Kammoun, Sana, Gdoura, Radhouane, Messadi-Akrout, Férièle
Format: Artigo
Sprache:Inglês
Veröffentlicht: Frontiers Media S.A. 2017
Schlagworte:
Microbiology
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC5725475/
https://ncbi.nlm.nih.gov/pubmed/29270157
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3389/fmicb.2017.02416
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