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Time-gated detection of protein-protein interactions with transcriptional readout

Transcriptional assays, such as yeast two-hybrid and TANGO, that convert transient protein-protein interactions (PPIs) into stable expression of transgenes are powerful tools for PPI discovery, screens, and analysis of cell populations. However, such assays often have high background and lose inform...

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Detalhes bibliográficos
Publicado no:eLife
Main Authors: Kim, Min Woo, Wang, Wenjing, Sanchez, Mateo I, Coukos, Robert, von Zastrow, Mark, Ting, Alice Y
Formato: Artigo
Idioma:Inglês
Publicado em: eLife Sciences Publications, Ltd 2017
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5708895/
https://ncbi.nlm.nih.gov/pubmed/29189201
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.7554/eLife.30233
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