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Determining rotational dynamics of the guanidino group of arginine side chains in proteins by carbon-detected NMR

Arginine residues are imperative for many active sites and protein-interaction interfaces. A new NMR-based method is presented to determine the rotational dynamics around the N(ε)–C(ζ) bond of arginine side chains. An application to a 19 kDa protein shows that the strengths of interactions involving...

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Veröffentlicht in:Chem Commun (Camb)
Hauptverfasser: Gerecht, Karola, Figueiredo, Angelo Miguel, Hansen, D. Flemming
Format: Artigo
Sprache:Inglês
Veröffentlicht: Royal Society of Chemistry 2017
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Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC5708338/
https://ncbi.nlm.nih.gov/pubmed/28840203
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1039/c7cc04821a
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