STRT-seq-2i: dual-index 5ʹ single cell and nucleus RNA-seq on an addressable microwell array

Single-cell RNA-seq has become routine for discovering cell types and revealing cellular diversity, but archived human brain samples still pose a challenge to current high-throughput platforms. We present STRT-seq-2i, an addressable 9600-microwell array platform, combining sampling by limiting dilut...

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Detalhes bibliográficos
Publicado no:Sci Rep
Main Authors: Hochgerner, Hannah, Lönnerberg, Peter, Hodge, Rebecca, Mikes, Jaromir, Heskol, Abeer, Hubschle, Hermann, Lin, Philip, Picelli, Simone, La Manno, Gioele, Ratz, Michael, Dunne, Jude, Husain, Syed, Lein, Ed, Srinivasan, Maithreyan, Zeisel, Amit, Linnarsson, Sten
Formato: Artigo
Idioma:Inglês
Publicado em: Nature Publishing Group UK 2017
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Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5703850/
https://ncbi.nlm.nih.gov/pubmed/29180631
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41598-017-16546-4
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