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Combinatorial metabolic engineering using an orthogonal tri-functional CRISPR system
Designing an optimal microbial cell factory often requires overexpression, knock-down, and knock-out of multiple gene targets. Unfortunately, such rewiring of cellular metabolism is often carried out sequentially and with low throughput. Here, we report a combinatorial metabolic engineering strategy...
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| Publicado no: | Nat Commun |
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| Main Authors: | , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Nature Publishing Group UK
2017
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5700065/ https://ncbi.nlm.nih.gov/pubmed/29167442 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41467-017-01695-x |
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