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Observing the fate of tRNA and its modifications by nucleic acid isotope labeling mass spectrometry: NAIL-MS

RNA in yeast, especially rRNA and tRNA are heavily modified to fulfill their function in protein translation. Using biosynthetic stable isotope labeled internal standards we quantified 12 modified nucleosides in tRNA from S. cerevisiae over 24 hours. We observed different quantities of modified nucl...

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Detalles Bibliográficos
Publicado en:RNA Biol
Main Authors: Heiss, Matthias, Reichle, Valentin F., Kellner, Stefanie
Formato: Artigo
Idioma:Inglês
Publicado: Taylor & Francis 2017
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Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC5699550/
https://ncbi.nlm.nih.gov/pubmed/28488916
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1080/15476286.2017.1325063
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