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A streamlined cloning workflow minimising the time-to-strain pipeline for Pichia pastoris

Although recent advances in E. coli self-assembly have greatly simplified cloning, these have not yet been harnessed for the high-throughput generation of expression strains in the early research and discovery phases of biopharmaceutical production. Here, we have refined the technique and incorporat...

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Detalles Bibliográficos
Publicado en:Sci Rep
Main Authors: Royle, Kate E., Polizzi, Karen
Formato: Artigo
Idioma:Inglês
Publicado: Nature Publishing Group UK 2017
Assuntos:
Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC5693959/
https://ncbi.nlm.nih.gov/pubmed/29150665
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41598-017-16172-0
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