Capturing an initial intermediate during the P450nor enzymatic reaction using time-resolved XFEL crystallography and caged-substrate

Time-resolved serial femtosecond crystallography using an X-ray free electron laser (XFEL) in conjunction with a photosensitive caged-compound offers a crystallographic method to track enzymatic reactions. Here we demonstrate the application of this method using fungal NO reductase, a heme-containin...

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Publicado no:Nat Commun
Main Authors: Tosha, Takehiko, Nomura, Takashi, Nishida, Takuma, Saeki, Naoya, Okubayashi, Kouta, Yamagiwa, Raika, Sugahara, Michihiro, Nakane, Takanori, Yamashita, Keitaro, Hirata, Kunio, Ueno, Go, Kimura, Tetsunari, Hisano, Tamao, Muramoto, Kazumasa, Sawai, Hitomi, Takeda, Hanae, Mizohata, Eiichi, Yamashita, Ayumi, Kanematsu, Yusuke, Takano, Yu, Nango, Eriko, Tanaka, Rie, Nureki, Osamu, Shoji, Osami, Ikemoto, Yuka, Murakami, Hironori, Owada, Shigeki, Tono, Kensuke, Yabashi, Makina, Yamamoto, Masaki, Ago, Hideo, Iwata, So, Sugimoto, Hiroshi, Shiro, Yoshitsugu, Kubo, Minoru
Formato: Artigo
Idioma:Inglês
Publicado em: Nature Publishing Group UK 2017
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Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5691058/
https://ncbi.nlm.nih.gov/pubmed/29147002
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41467-017-01702-1
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