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Monitoring Intracellular pH change with a Genetically Encoded and Ratiometric Luminescence Sensor in Yeast and Mammalian Cells

“pHlash” is a novel bioluminescence-based pH sensor for measuring intracellular pH, which is developed based on Bioluminescence Resonance Energy Transfer (BRET). pHlash is a fusion protein between a mutant of Renilla luciferase (RLuc) and a Venus fluorophore. The spectral emission of purified pHlash...

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Bibliografiske detaljer
Udgivet i:Methods Mol Biol
Main Authors: Zhang, Yunfei, Robertson, J. Brian, Xie, Qiguang, Johnson, Carl Hirschie
Format: Artigo
Sprog:Inglês
Udgivet: 2016
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC5687249/
https://ncbi.nlm.nih.gov/pubmed/27424899
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/978-1-4939-3813-1_9
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