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pHlash: A New Genetically Encoded and Ratiometric Luminescence Sensor of Intracellular pH
We report the development of a genetically encodable and ratiometic pH probe named “pHlash” that utilizes Bioluminescence Resonance Energy Transfer (BRET) rather than fluorescence excitation. The pHlash sensor–composed of a donor luciferase that is genetically fused to a Venus fluorophore–exhibits p...
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| Main Authors: | , , , |
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| Format: | Artigo |
| Language: | Inglês |
| Published: |
Public Library of Science
2012
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| Subjects: | |
| Online Access: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3419163/ https://ncbi.nlm.nih.gov/pubmed/22905204 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0043072 |
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